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KMID : 1024520070160101203
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Journal of the Environmental Sciences
2007 Volume.16 No. 10 p.1203 ~ p.1208
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Production of Keratinolytic Protease by Bacillus pumilus RS7 and Feather Hydrolysate As a Source of Amino Acids
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Woo Eun-Ok
Kim Min-Ju
Son Hyeng-Sik
Ryu Eun-Yeon
Jeong Seong-Yun
Son Hong-Joo
Lee Sang-Joon
Park Geun-Tae
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Abstract
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Feathers are produced in huge quantities as a waste product at commercial poultry processing plants. Since feathers are almost pure keratin protein, feather wastes represent an alternative to more expensive dietary ingredients for animal feedstuffs. Generally they become feather meal used as animal feed after undergoing physical and chemical treatments. These processes require significant energy and also cause environmental pollutions. Therefore, biodegradation of feather by microorganisms represents an alternative method to prevent environment contamination. The aim of this study was to investigate cultural conditions affecting keratinolytic protease production by Bacillus pumilus RS7. We also assessed the nutritive value of microbial and alkaline feather hydrolysates, The composition of optimal medium for the keratinolytic protease was fructose 0.05%, yeast extract 0.3%, NaCl 0.05%, K2HPO4 0.03%, KH2PO4 0.04% and MgCl2 6H2O 0.01%, respectively. The optimal temperature and initial pH was and 9.0, respectively. The keratinolytic protease production under optimal condition reached a maximum after 18 h of cultivation. Total amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was and , respectively. Essential amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was and , respectively. Thus, feather hydrolysates have the potential for utilization as an ingredient in animal feed.
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KEYWORD
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Amino acid, Chicken feather, Feather hydrolysate, Keratin, Keratinolysis
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